File:A-Novel-Chemotaxis-Assay-in-3-D-Collagen-Gels-by-Time-Lapse-Microscopy-pone.0052251.s001.ogv

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A-Novel-Chemotaxis-Assay-in-3-D-Collagen-Gels-by-Time-Lapse-Microscopy-pone.0052251.s001.ogv ‎(Ogg Theora video file, length 12 s, 336 × 238 pixels, 85 kbps)

Summary[edit]

Description
English: Directional migration of freshly isolated human neutrophils in 3D collagen matrix towards Il-8. Images were taken at 10× magnification, and correspond to the Z projection of 5 layers reported at a reduced time and spatial resolution with about one frame every two minutes, in order to minimize the file dimension. The first 30 minutes, where each frame has a white overlay showing the progressive time and a dimension bar, are representative of the unstimulated condition where a limited fraction of cells move randomly. The IL-8 solution was added to the reservoir at t = 30 min. In the second part of the movie where the overlay is in black, cells are under the effect of an IL-8 concentration gradient. An increase in cell motility, that has been quantified in terms of average velocity and fraction of motile cells, and a preferential movement toward the source of the chemoattractant, i.e. towards the bottom part of the image, can be clearly noticed.
Date
Source Movie S1 from Vasaturo A, Caserta S, Russo I, Preziosi V, Ciacci C, Guido S (2012). "A Novel Chemotaxis Assay in 3-D Collagen Gels by Time-Lapse Microscopy". PLOS ONE. DOI:10.1371/journal.pone.0052251. PMID 23284956. PMC: 3526591.
Author Vasaturo A, Caserta S, Russo I, Preziosi V, Ciacci C, Guido S
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Author Vasaturo A, Caserta S, Russo I, Preziosi V, Ciacci C, Guido S
Image title Directional migration of freshly isolated human neutrophils in 3D collagen matrix towards Il-8. Images were taken at 10× magnification, and correspond to the Z projection of 5 layers reported at a reduced time and spatial resolution with about one frame every two minutes, in order to minimize the file dimension. The first 30 minutes, where each frame has a white overlay showing the progressive time and a dimension bar, are representative of the unstimulated condition where a limited fraction of cells move randomly. The IL-8 solution was added to the reservoir at t 
Software used Xiph.Org libtheora 1.1 20090822 (Thusnelda)
Usage terms http://creativecommons.org/licenses/by/3.0/
Date and time of digitizing 2012
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