File:A-mTurquoise-Based-cAMP-Sensor-for-Both-FLIM-and-Ratiometric-Read-Out-Has-Improved-Dynamic-Range-pone.0019170.s001.ogv

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A-mTurquoise-Based-cAMP-Sensor-for-Both-FLIM-and-Ratiometric-Read-Out-Has-Improved-Dynamic-Range-pone.0019170.s001.ogv ‎(Ogg Theora video file, length 5.0 s, 750 × 420 pixels, 353 kbps)

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English: Increase in fluorescence lifetime of TEpacVV upon cAMP-induced sensor unfolding. The fluorescence lifetime of the mTurquoise donor in TEpacVV is strongly increased when intracellular cAMP levels are raised with IBMX/forskolin. The analysis of the FLIM time-lapse experiment shows (upper row from left to right), mTurquoise intensity, phase lifetime map, phase lifetime map overlaid with the intensity, a histogram depicting the phase lifetime distribution, and (lower row from left to right) modulation lifetime map, modulation lifetime map overlaid with the intensity, a histogram depicting the modulation lifetime distribution. The lifetime maps are color-coded according to the LUT shown in the histogram and the lifetime/intensity overlays according to that shown in the lower left corner. Quantification of the fluorescence lifetime, as measured by frequency domain FLIM, over time is shown in figure 1D of the manuscript.
Date
Source Movie S1 from Klarenbeek J, Goedhart J, Hink M, Gadella T, Jalink K (2011). "A mTurquoise-Based cAMP Sensor for Both FLIM and Ratiometric Read-Out Has Improved Dynamic Range". PLOS ONE. DOI:10.1371/journal.pone.0019170. PMID 21559477. PMC: 3084777.
Author Klarenbeek J, Goedhart J, Hink M, Gadella T, Jalink K
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Author Klarenbeek J, Goedhart J, Hink M, Gadella T, Jalink K
Image title Increase in fluorescence lifetime of TEpacVV upon cAMP-induced sensor unfolding. The fluorescence lifetime of the mTurquoise donor in TEpacVV is strongly increased when intracellular cAMP levels are raised with IBMX/forskolin. The analysis of the FLIM time-lapse experiment shows (upper row from left to right), mTurquoise intensity, phase lifetime map, phase lifetime map overlaid with the intensity, a histogram depicting the phase lifetime distribution, and (lower row from left to right) modulation lifetime map, modulation lifetime map overlaid with the intensity, a histogram depicting the modulation lifetime distribution. The lifetime maps are color-coded according to the LUT shown in the histogram and the lifetime/intensity overlays according to that shown in the lower left corner. Quantification of the fluorescence lifetime, as measured by frequency domain FLIM, over time is shown in figure 1D of the manuscript.
Software used Xiph.Org libtheora 1.1 20090822 (Thusnelda)
Usage terms http://creativecommons.org/licenses/by/3.0/
Date and time of digitizing 2011
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